Name: Dong Wang
Title: Professor
Major: Animal Genetic breeding and reproduction
Research direction: Animal reproduction
Contact number: 010-62879492
E-mail： wangdong02@caas.cn; dwangcn2002@vip.sina.com
Personal Information
Wang Dong, native of Chifeng, Inner Mongolia, Doctor, Pofessor and doctoral supervisor of animal Genetics, breeding and reproduction. In 1990, he received a Bachelor's degree in Animal Science from Jerimu husbandry College. In 2000, he received a master's degree in Animal Genetics, Breeding and Reproduction from Jilin Agricultural University. In 2003, he received his Doctor degree in Animal Genetics, Breeding and Reproduction from Chinese Agricultural University. From 2004 to 2005, he was a visiting scholar at the Piedmont Cattle Breeding Center (ANABORAPI) and the University of Turin. Since 2003, he has been working at the Institute of Animal Science(IAS), Chinese Academy of Agricultural Sciences(CAAS). He is a member of the sixth Academic Committee of IAS of CAAS, a backbone expert of the Genetic engineering and germplasm Innovation team, mainly engaged in animal reproduction research; an expert of dairy cow reproductive regulation in Beijing Livestock Innovation Team; the Secretary-General of the Animal Reproduction Branch of the Chinese Association of Animal Science and Veterinary Medicine(CAAV); Vice Chairman of breeding Committee of China Dairy Association.
Scientific research projects
Has presided over the national "十一五" 863 plan, "十二五" science and technology support plan, National Natural Science Fund and other more than 10 national projects, mainly include:
2.1 "863" project "X, Y sperm transcriptome and proteome level gene differential expression" (2006AA10Z143, 2006.12 ~2010.10);
2.2 National Natural Science Foundation Project "Molecular Basis of Bovine Sperm sex-specific Protein by Comparative Proteomic Methods" (30671502,2007.01 ~2009.12);
2.3 the 12th Five-Year science and technology support project "Research and demonstration of high-efficiency superplatoon and cloned embryo production technology of cattle and sheep" (2011BAD19B02,2011.01-2013.12);
2.4 National Natural Science Foundation Project "Study on gene expression mechanism of sperm cell deformation in Cows after Meiosis by applying LCM combined with RNA-Seq Technology" (31372296, 2014.01-2017.12)
2.5 The National Natural Science Foundation project "Study on the relationship between SSCs and SCs Toxicity Regulation after testicular injection of busulfan" (31772595, 2018.01~2021.12);
2.6 National Natural Science Foundation of China (NSFC) Project "Study on the Variation of Quiet estrus Body Temperature of Holstein Cows and its Hormone Regulation Mechanism" (32072739, 2021.01.01-2024.12.31).
Research direction and achievements
In view of the industrial problems such as difficult identification of cow estrus, heavy workload, and difficult detection of estrus at night, the automatic system of cow breeding management was successfully developed based on the activity index, and the automatic identification of estrus and pregnancy diagnosis were realized, and the detection rate of estrus and the detection rate of the first estrus after mating was more than 80%. The automatic monitoring technology of cow vaginal temperature was developed, and it was found that the activity did not significantly increase while the vaginal temperature of hot cows increase for a long time, so the automatic identification technology of cow estrus was developed to break the bottleneck of quiet estrus identification technology. Related research has published more than 10 scientific research papers, won 2 national invention patents, 1 international patent, and 8 utility model patents. Greatly improve the breeding efficiency of cattle farms, and save more than 200,000 yuan per year for demonstration cattle farms.
In response to the problems of high toxicity and poor effect in the preparation of spermatogonia stem cell transplantation recipient, the study revealed the toxic mechanism of busulfan breaking through the blood-testis barrier, activating inflammatory factors, inducing orchitis and damaging spermatogenic cells, and found that adenosine diphospho-ribosyl transferase 3 (ART3) played an important role in spermatogenesis and deformation. The isolation and culture of mouse spermatogonial stem cells and the preparation and transplantation of recipient were established, and the donor mouse offspring were obtained after transplanted, which laid a safe and technical foundation for the preparation of efficient surrogate fathers in domestic animals. Related research has published more than 10 scientific research papers, and won 1 national invention and utility model patents.
To solve the problem of long PCR detection time and unstable effect, the two-temperature gradient rapid PCR technology was developed, which can complete PCR amplification in about 50 minutes, and based on this, the rapid detection of bovine spinal malformation syndrome, rapid identification of embryo sex, rapid evaluation of X/Y sperm purity and efficient PCR detection of double muscle genes in beef were established. It has provided technical support for marker-assisted breeding and sex control, and has published more than 30 related research papers, obtained 6 invention patents, and has a number of technology transformation applications. Among them, dairy cow embryo sex identification technology as an important part of the "Demonstration and promotion of efficient and safe Dairy cow breeding technology" project, won the second prize of Beijing Agricultural Technology promotion (2013-2-14).
4. Representative papers and patents
4.1 Representative patents:
A novel rapid sex identification method for cattle and their early embryos (ZL201010182747.5);
A preparation method for spermatogonial stem cell transplantation recipient (ZL 201210343103.9);
Rapid detection of single base mutation site of the third exon of myostatin gene (ZL200910242827.2);
Method for detecting the separation purity of bovine X and Y sperm, invention patent (Patent No. 201010182763.4);
A method for predicting bovine estrus by detecting bovine vaginal temperature (ZL201711116395.1);
Method for reporting bovine estrus (PCT/CN2018/088839).
4.2 Representative articles:
Lingjun Zhao, Jing Zhao, Zhihao Dong, Shiyuan Xu, Dong Wang*. Mechanisms underlying impaired spermatogenic function in orchitis induced by busulfan, Reproductive Toxicology 115 (2023) 1-7. https://doi.org/10.1016/j.reprotox.2022.11.002.
Li Xin, Duan Chenying, Li Ruyi and Wang Dong*. Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies, Animals（Basel），2021, 11(1):80. doi: 10.3390/ani11010080.
Tian Hongzhi, Liu Jiangjing, Chen Xiaoli, Li Shujing, Li Xin, Mengal Kifayatullah, Lu Yongqiang, Wang Dong*. Effects of ambient temperature and humidity on body temperature and activity of heifers, and a novel idea of heat stress monitoring, Animal Production Science 2021, https://doi.org/10.1071/AN20156.
Wang shuilian, Zhang hongliang, Tian hongzhi, Chen xiaoli, Lishujing, Lu yongqiaong, Lilanqi, Wang dong*. Alterations in vaginal temperature during the estrous cycle in dairy cows detected by a new intravaginal device——a pilot study. Tropical Animal Health and Production, 2020, 52(5), 2265-2271
Liu Jiangjing, Li Lanqi, Chen Xiaoli, Lu Yongqiang, Wang Dong*. Effects of heat stress on body temperature, milk production, and reproduction in dairy cows: a novel idea for monitoring and evaluation of heat stress—A review, Asian-Australasian Journal of Animal Sciences, 2019, 32(9):1332-1339.
Chen Xiaoli, Liang Mingyuan, Wang Dong*. Progress on the study of the mechanism of busulfan cytotoxicity. Cytotechnology, 2018, 70:497-502.
Kou Hongxiang, Zhao Yiqiang, Chen Xiaoli, Wang Dong*. The study on automatic measurement of the surface temperature of cattle and its fitting with rectal temperature, PLoS ONE, 2017,12(4): e0175377. https://doi.org/10.1371/journal.pone.0175377.
Ren Xiaoxia, Chen Xiaoli, Wang Zhenling, Wang Dong*. Is transcription in mature sperm stationary or dynamic? Journal of Reproduction and Development, 2017, 10，63(5): 439~443.
Zuo Haiyang, Zhang Junfang, Zhang Liuguang, Ren Xiaoxia, Chen Xiaoli, Hao Haisheng, Zhao Xueming, Wang Dong*.Transcriptomic variation during spermiogenesis in mouse germ cells, PLoS One. 2016,11(11):e0164874.
Qin Yusheng, Liu Ling, He Yanan, Ma Wenzhi, Zhu Huabin, Liang Mingyuan, Hao Haisheng, Qin Tong, Zhao Xueming, Wang Dong*. Testicular injection of busulfan for recipient preparation in transplantation of spermatogonial stem cells in mice, Reproduction, Fertility and Development, 2016,28,1916-1925.
Chen Xiaoli, Wang Yonggui, Zhu Huabin, Hao H, Zhao Xueming, Qin Tong, Wang Dong*. Comparative transcript profiling of gene expression of fresh and frozen-thawed bull sperm. Theriogenology. 2015 Mar 1;83(4):504-11.
Chen Xiaoli, Yue Yang, He Yanan, Zhu Huabin, Hao Haisheng, Zhao Xueming, Qin Tong, Wang Dong*. Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray. Mol Reprod Dev. 2014 Oct;81(10):908-17.
Chen Xiaoli, Zhu Huabin, Hu Chuanhuo, Hao Haisheng, Zhang Junfang, Zhao Xueming, Zhu Huishan, Du Weihua, Qin Tong, Liu Yan, Zhao Kan, Wang Dong*. Identification of Differentially Expressed Proteins Between Fresh and Frozen-Thawed Boar Spermatozoa by iTRAQ-Coupled 2D LC-MS/MS, Reproduction. 2014, 147:321-330.