职称：研究员

专业：生物化学与分子生物学

研究方向：蛋白智能设计

联系电话：010-62599910

 E-mail： tianjian@caas.cn

一、个人信息

田健，1979年4月生，河北省新乐人，博士，研究员，博士生导师。2003年获河北农业大学学士学位；2006年获中国农业科学院硕士学位；2011年获中国农业科学院博士学位；2013年-2014年在美国哈佛大学从事博士后研究。2023年至今在中国农业科学院北京畜牧兽医研究所工作。主要从事蛋白智能分子设计研究，现任微生物与酶工程创新团队骨干专家；中国生物信息学会（筹）农林专业委员会委员。入选国家级人才计划，荣获昇腾AI创新大赛区域赛金奖和全国赛银奖，获第四届范云六院士青年科技创新奖。

二、研究方向与成果

2006年以来，主要从事蛋白智能分子设计研究工作，致力于构建人工智能与酶蛋白相结合的蛋白智能分子设计平台，提高酶蛋白的稳定性、表达量、催化活性等应用性能，提升其产业价值。

三、在研项目

1. 国家自然科学基金重大项目“节粮高效智能化育种技术体系建立与应用”（编号：32494804）

2. “十四五”国家重点研发计划政府间国际科技创新合作重点专项项目负责人（编号：2022YFE0135300）

3. “十四五”国家重点研发计划“造纸酶制剂的智造与产业化”课题负责人（编号：2022YFC2105501）

四、代表性论文

1.Xu, Guoshun, Li, Suzhen, Li, Haoyu, Ren, Xiaopu, Ding, Yekun, Pang, Xinli, Liu, Xu, Tang, Qiaoling, Tu, Tao, Wang, Yuan, Luo, Huiying, Yao, Bin, Tian, Jian*(Corresponding author), Chen, Rumei* and Guan, Feifei*: CasMiner: a deep-learning tool for high-throughput mining and rational design of efficient Cas9. National Science Review 2026, 13(6).

2.Han Gao*, Feifei Guan*, Boyu Luo*, Dongdong Zhang*, Wei Liu*, Yuying Shen, Lingxi Fan, Guoshun Xu, Yuan Wang, Tao Tu, Ningfeng Wu, Bin Yao, Huiying Luo*, Yue Teng*, Jian Tian*(Corresponding author), Huoqing Huang*: DLFea4AMPGen de novo design of antimicrobial peptides by integrating features learned from deep learning models. Nature Communications 2025, 16(1):9134.

3.Zhe Wang, Tuoyu Liu, Guoshun Xu, Han Gao, Ruohan Zhang, Honglian Zhang, Guijie Zhang, Ningfeng Wu, Bin Yao, Huiying Luo,*, Feifei Guan*, Jian Tian*(Corresponding author): MPCutter: Predicting Protease-specific Substrate Cleavage Sites Using a Protein Language Model. Genomics Proteomics Bioinformatics 2025. doi: 10.1093/gpbjnl/qzaf130

4.Tuoyu Liu, Yiyang Zhang, Yanjun Li, Guoshun Xu, Han Gao, Pengtao Wang, Tao Tu, Huiying Luo, Ningfeng Wu, Bin Yao, Bo Liu*, Feifei Guan*, Huoqing Huang*, Jian Tian*(Corresponding author). Effective gene expression prediction and optimization from protein sequences. Advanced Science. 2025. DOI: 10.1002/advs.202407664.

5.Miao, Ruiju, Xu, Guoshun, Ding, Yekun, Ding, Zundan, Woodard, J., Tu, Tao, Luo, Huiying., Wu, Ningfeng, Yao, Bin, Guan, Feifei*, Tian, Jian*(Corresponding author). Engineering dual-functional and thermophilic BMHETase for efficient degradation of polyethylene terephthalate. Bioresour Technol 2024. 414:131556.

6.Liu, Hanqing#, Guan, Feifei#, Liu, Tuoyu#, Yang, Lixin#, Fan, Lingxi, Liu, Xiaoqing, Luo, Huiying, Wu, Ningfeng, Yao, Bin, Tian, Jian*(Corresponding author), Huang, Huoqing*: MECE: a method for enhancing the catalytic efficiency of glycoside hydrolase based on deep neural networks and molecular evolution. Science Bulletin 2023, 68:2793-2805

7.Liu, Tuoyu, Gao, Han, Ren, Xiaopu, Xu, Guoshun, Liu, Bo, Wu, Ningfeng, Luo, Huiying, Wang, Yuan, Tu Tao, Yao, Bin, Huang, Huoqing*, Tian, Jian*(Corresponding author): Protein-protein interaction and site prediction using transfer learning. Brief Bioinform 2023, 24(6).

8.Ding, Zundan#, Xu, Guoshun#, Miao, Ruiju, Wu, Ningfeng, Zhang, Wei, Yao, Bin, Guan, Feifei, Huang, Huoqing*, Tian, Jian*, 2023. Rational redesign of thermophilic PET hydrolase LCCICCG to enhance hydrolysis of high crystallinity polyethylene terephthalates. J Hazard Mater 453, 131386. https://doi.org/10.1016/j.jhazmat.2023.131386.

9.Guan, Feifei#; Han, Yanshuo#; Yan, Kai; Zhang, Yan; Zhang, Zhifang; Wu, Ningfeng*; Tian, Jian*(Corresponding author); Highly efficient production of chitooligosaccharides by enzymes mined directly from the marine metagenome., Carbohydrate Polymers, 2020, 234: 115909.

10.Tian, Jian#，Woodard, Jaie#，Whitney, Anna，Shakhnovich, Eugene(*)，Thermal Stabilization of Dihydrofolate Reductaseusing Monte Carlo Unfolding Simulations and its Functional Consequences，PLoS Computational Biology, 2015, 2015, 11(4):e1004207.