一、研究方向

　　1、技术平台建设与病原致病机制研究

　　围绕重要宠物疫病诊断、防控与免疫机制等核心科学问题，以宠物重要疫病为研究对象，针对病毒、细菌、寄生虫类宠物疫病防治中的重大科学问题和关键技术，开展病原学、病原与宿主、病原与环境相互作用规律的研究。构建了狂犬病病毒、犬细小病毒、犬瘟热病毒等反向遗传操作平台，为宠物疫病的基础研究和应用研究奠定基础。

　　2、宠物疫病诊断与防控技术研究

　　针对宠物病毒病、细菌病、寄生虫病等宠物疫病防治中的急需的诊断产品、疫苗和治疗性制剂进行研制和开发。开展了犬细小病毒病-犬瘟热等二联、三联疫苗的开发研究；开展以了狂犬病毒、犬瘟热病毒为载体的基因工程疫苗研究和评价；细小病毒亚单位疫苗研究和安全评价。澄清了我国犬瘟热、犬细小病毒病、猫瘟热等宠物犬猫主要传染病病原流行和发生规律，建立犬猫重要传染病新型、快速诊断和鉴别诊断技术平台。

　　二、代表性成果

　　1.    Zhang L, Sun M, Zhang Q, Wang J, Cao Y, Cui S, Su J: Long-term passage of duck Tembusu virus in BHK-21 cells generates a completely attenuated and immunogenic population with increased genetic diversity. Vaccine 2019.

　　2.    Zhang L, Liang R, Zhang G, Zhai Z, Deng Y, Li J, Cui S: Analysis of the microRNA expression profiles in feline kidney cell line infected with feline panleukopenia virus. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2019, 75:103945.

　　3.    Zhang L, Gao D, Yu Y, Liu Y, Liu W, Li J, Cui S: Establishment of a rescue system for porcine parvovirus using a seamless cloning method. Archives of virology 2019, 164(5):1459-1467.

　　4.    Tang X, Wang C, Liang L, Hu D, Zhang S, Duan C, Suo J, Liu X, Suo X, Cui S: Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection. Parasites & vectors 2019, 12(1):347.

　　5.    Liu C, Liu Y, Liang L, Cui S, Zhang Y: RNA-Seq based transcriptome analysis during bovine viral diarrhoea virus (BVDV) infection. BMC genomics 2019, 20(1):774.

　　6.    Liu C, Li X, Liang L, Li J, Cui S: Isolation and phylogenetic analysis of an emerging Senecavirus A in China, 2017. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2019, 68:77-83.

　　7.    Leal E, Liu C, Zhao Z, Deng Y, Villanova F, Liang L, Li J, Cui S: Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China. Viruses 2019, 11(6).

　　8.    Zhang L, Luo Y, Liang L, Li J, Cui S: Phylogenetic analysis of porcine circovirus type 3 and porcine circovirus type 2 in China detected by duplex nanoparticle-assisted PCR. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2018, 60:1-6.

　　9.    Wang J, Luo Y, Liang L, Li J, Cui S: A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection. Archives of virology 2018, 163(12):3345-3349.

　　10.  Zhu Y, Liang L, Luo Y, Wang G, Wang C, Cui Y, Ai X, Cui S: A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens. Virus genes 2017, 53(1):71-76.

　　11.  Xi J, Zhang Y, Wang J, Yu Y, Zhang X, Li Z, Cui S, Liu W: Generation of an infectious clone of AMDV and identification of capsid residues essential for infectivity in cell culture. Virus research 2017, 242:58-65.

　　12.  Luo YK, Liang L, Tang QH, Zhou L, Shi LJ, Cong YY, Lin WC, Cui SJ: Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China. Scientific reports 2017, 7(1):438.

　　13.  Huang L, Xiong T, Yu H, Zhang Q, Zhang K, Li C, Hu L, Zhang Y, Zhang L, Liu Q et al: Encephalomyocarditis virus 3C protease attenuates type I interferon production through disrupting the TANK-TBK1-IKKepsilon-IRF3 complex. The Biochemical journal 2017, 474(12):2051-2065.

　　14.  Zhu Y, Wang GH, Cui YD, Cui SJ: Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus. Archives of virology 2016, 161(9):2543-2547.

　　15.  Zhou L, Tang Q, Shi L, Kong M, Liang L, Mao Q, Bu B, Yao L, Zhao K, Cui S et al: Full-length genomic characterization and molecular evolution of canine parvovirus in China. Virus genes 2016, 52(3):411-416.

　　16.  Yu H, Huang L, Zhang Y, Hu L, Wang S, Li J, Cai X, Cui S, Weng C: An attenuated EMCV-HB10 strain acts as a live viral vector delivering a foreign gene. The Journal of general virology 2016, 97(9):2280-2290.

　　17.  Yin X, Qi Y, Ren M, Wang S, Jiang H, Feng H, Cui S: Roscovitine treatment caused impairment of fertilizing ability in mice. Toxicology letters 2015, 237(3):200-209.

　　18.  Luo Y, Liang L, Zhou L, Zhao K, Cui S: Concurrent infections of pseudorabies virus and porcine bocavirus in China detected by duplex nanoPCR. Journal of virological methods 2015, 219:46-50.

　　19.  Huang L, Liu Q, Zhang L, Zhang Q, Hu L, Li C, Wang S, Li J, Zhang Y, Yu H et al: Encephalomyocarditis Virus 3C Protease Relieves TRAF Family Member-associated NF-kappaB Activator (TANK) Inhibitory Effect on TRAF6-mediated NF-kappaB Signaling through Cleavage of TANK. The Journal of biological chemistry 2015, 290(46):27618-27632.

　　三、创新团队成员