一、研究方向

　　1、家畜配子与胚胎发育调控研究

　　提高家畜繁殖效率，充分挖掘公母畜的繁殖潜能是家畜繁殖学的重要任务之一。然而，大多数家畜精、卵和胚胎体内外发育的调控机理尚不明晰，尤其是人为调控繁殖新技术的基础研发需要进一步提高。本团队将家畜精、卵与胚胎发育调控机制作为主要研究方向，重点研究配子、胚胎发育质量的影响因素和分子调控机制，旨在为精、卵和胚胎体外发育的调控提供新的靶位点，以提高动物胚胎的体内外生产效率，最大程度发挥家畜繁育效率。

　　2、家畜繁殖调控新技术研究与示范

　　家畜繁殖调控新技术涉及卵泡发育调控、配子保存、体内外胚胎生产等，是快速扩繁优质畜群、改良畜群遗传质量以及培育家畜新品种（系）的重要技术手段。本团队围绕繁殖调控新技术开发与推广开展系列研究，重点研究家畜精、卵和胚胎超低温保存机制和方法；X/Y精子分离与早期胚胎质量鉴定技术；国外繁殖新技术的引进、本土化创新和应用示范；活体采卵技术（OPU）-体外受精（IVF）技术体系；基因编辑技术在改良家畜肉质和提高抗病力等重要经济性状中的应用，以实现优秀种群规模化改良换代，加快良种扩繁速度。

　　二、代表性成果

　　1. Zou H, Yu D, Du X, Wang J, Chen L, Wang Y, Xu H, Zhao Y, Zhao S, Pang Y, Liu Y, Hao H, Zhao X, Du W, Dai Y, Li N, Wu S, Zhu H. No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas. Cell Mol Life Sci. 2019 Nov;76(22):4525-4538.

　　2. Li F, Cui L, Yu D, Hao H, Liu Y, Zhao X, Pang Y, Zhu H, Du W. Exogenous glutathione improves intracellular glutathione synthesis via the γ-glutamyl cycle in bovine zygotes and cleavage embryos. J Cell Physiol. 2019 May;234(5):7384-7394.

　　3. Pang YW, Jiang XL, Wang YC, Wang YY, Hao HS, Zhao SJ, Du WH, Zhao XM, Wang L, Zhu HB. Melatonin protects against paraquat-induced damage during in vitro maturation of bovine oocytes. J Pineal Res. 2019 Jan;66(1):e12532.

　　4.Zhao XM, Wang N, Hao HS, Li CY, Zhao YH, Yan CL, Wang HY, Du WH, Wang D, Liu Y, Pang YW, Zhu HB. Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events. J Pineal Res. 2018 Jan;64(1).

　　5. Zhang Y, Wang X, Jiang Q, Hao H, Ju Z, Yang C, Sun Y, Wang C, Zhong J, Huang J, Zhu H. DNA methylation rather than single nucleotide polymorphisms regulates the production of an aberrant splice variant of IL6R in mastitic cows. Cell Stress Chaperones. 2018 Jul;23(4):617-628.

　　6.Zhao SJ, Pang YW, Zhao XM, Du WH, Hao HS, Zhu HB. Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms. Oncotarget. 2017 Jan 17;8(3):4656-4667.

　　7. Pang YW, Sun YQ, Jiang XL, Huang ZQ, Zhao SJ, Du WH, Hao HS, Zhao XM, Zhu HB. Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development. Mol Reprod Dev. 2016 Nov;83(11):993-1002.

　　8. Wei R, Zhao X, Hao H, Du W, Zhu H. Embryonic stem-like cells from rabbit blastocysts cultured with melatonin could differentiate into three germ layers in vitro and in vivo. Mol Reprod Dev. 2016 Nov;83(11):1003-1014.

　　9. Shan-Jiang Zhao, Xue-Ming Zhao, Wei-Hua Du, Hai-Sheng Hao, Yan Liu, Tong Qin, Dong Wang, Hua-bin Zhu. Production of early monozygotic twin bovine embryos in vitro by the blastomere separation and coculture technique. Journal of Integrative Agriculture 2015, 14(10): 2034-2041.

　　10.Yu D, Zhang S, Du W, Zhang J, Fan Z, Hao H, Liu Y, Zhao X, Qin T, Zhu H. Expression of intracellular interferon-alpha confers antiviral properties in transfected bovine fetal fibroblasts and does not affect the full development of SCNT embryos. PLoS One. 2014 Jul 8;9(7):e94444.

　　三、创新团队成员