一、研究方向

　　1、苜蓿等重要饲草遗传育种研究

　　紫花苜蓿因营养价值丰富、适口性好，为分布最广、最具经济价值的豆科牧草。我国当前苜蓿育成品种数量少，引进的国外苜蓿品种因不适宜我国的土壤、气候特点，多表现出抗逆性、持久性差，易发生病虫害等特性，严重制约我国苜蓿产业的发展。本创新团队立足于已有的苜蓿遗传育种研究工作基础，结合苜蓿产业生产实际需求，采用常规育种技术与分子育种技术结合的方法，开展苜蓿种质创新和新品种选育，培育自主创新的抗逆、高产、优质苜蓿新品种。同时开展苜蓿重要性状分子标记辅助育种技术、苜蓿重要性状关键基因调控机制及苜蓿多基因组装育种技术的创新研究，并进一步开展相关基础理论研究，为我国苜蓿产业及草食畜牧业高效可持续发展提供技术支撑和理论依据。

　　2、饲草高效栽培技术研究

　　水分和养分是植物生长过程必需的物质，其耦合作用机理和吸收规律是牧草栽培管理的重要研究内容。本团队开展了紫花苜蓿、饲用燕麦等重要饲草养分平衡机理研究，研究水肥耦合机制和栽培管理技术；利用多年多点的原位控制肥效试验、同位素示踪和分子生物学技术，对土壤养分、植物组织营养和牧草生产性能之间的关系以及主要养分的吸收规律进行深入、系统的研究，初步形成了牧草的营养诊断和推荐施肥技术体系。另外，逆境是指植物的非正常生长环境，是影响植物产量和品质的重要环境因素，植物在生长过程中为对抗逆境胁迫进化出一系列的生理生态变化策略。本团队开展了苜蓿等重要饲草在干旱、盐碱、低磷、砂性土壤等逆境胁迫下的生理生态响应机制和抗逆性评价，开展苜蓿耐旱、耐低磷等逆境的分子机制研究。

　　二、代表性成果

　　1、育成品种与奖励

　　育成耐盐苜蓿新品种中苜1号、中苜3号、中苜5号，育成高产苜蓿新品种中苜2号、中苜4号、中苜9号，早熟高产苜蓿新种中苜7号等9个品种。获北京市科技进步二等奖1项；获中国农业科学院科技进步一等奖1项；获内蒙古自治区科技进步二等奖1项；获全国农牧渔业丰收奖一等奖1项；其他省部级奖项2项。

　　2、发表文章

　　1. Gao Yanli, Li Mingna, Zhang Xiaxiang, Yang Qingchuan, Huang Bingru*. Upregulation of lipid metabolism and glycine betaine synthesis are associated with choline‐induced salt tolerance in halophytic seashore paspalum[J]. Plant, Cell & Environment, 2019.

　　2. Gao Yanli, Long Ruicai, Kang Junmei, Wang Zhen, Zhang Tiejun, Sun Hao, Li Xiao, Yang Qingchuan*. Comparative proteomic analysis reveals that antioxidant system and soluble sugar metabolism contribute to salt tolerance in alfalfa (Medicago sativa L.) leaves[J]. Journal of proteome research, 2018, 18(1): 191-203.

　　3. Sun Hao, Long Ruicai, Zhang Fan, Zhang Tiejun, Kang Junmei, Wang Zhen*, Cao Chunyu, Yu Jie, Yang Qingchuan*. Proteomic Analysis of Shoot Tips from Two Alfalfa Cultivars with Different Florescence[J]. Plant Molecular Biology Reporter, 2019.

　　4. Kang Junmei, Zhang Qiaoyan, Jiang Xu, Zhang Tiejun, Long Ruicai, Yang Qingchuan, Wang Zhen*. Molecular Cloning and Functional Identification of a Squalene Synthase Encoding Gene from Alfalfa (Medicago sativa L.)[J]. International journal of molecular sciences, 2019, 20(18): 4499.

　　5. Cao Chunyu, Long Ruicai, Zhang Tiejun, Kang Junmei, Wang Zhen, Wang Pingqing, Sun Hao, Yu Jie, Yang Qingchuan*. Genome-Wide Identification of microRNAs in Response to Salt/Alkali Stress in Medicago truncatula through High-Throughput Sequencing[J]. International Journal of Molecular Sciences, 2018, 19(12).

　　6. Gao Yanli, Cui Yanjun, Long Ruicai, Sun Yan, Zhang Tiejun, Yang Qingchuan*, Kang Junmei. Salt-stress induced proteomic changes of two contrasting alfalfa cultivars during germination stage[J]. Journal of the Science of Food and Agriculture, 2018.

　　7. Wang Zhen, Wang Xiaomin, Xie Bo, Hong Zonglie, Yang Qingchuan*. Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) regulates cell cycling potentially by cooperating with nucleosome assembly protein AtNAP1; 1[J]. BMC plant biology, 2018, 18(1): 99.

　　8. Long Ruicai, Gao Yanli, Sun Hao, Zhang Tiejun*, Li Xiao, Li Mingna, Sun Yan, Kang Junmei, Wang Zhen, Ding Wang, Yang Qingchuan*. Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species[J]. Scientific reports, 2018, 8(1): 9553.

　　9. Long Ruicai, Sun Hao, Cao Chunyu, Zhang Tiejun, Kang Junmei, Wang Zhen, Li Mingna, Gao Yanli, Li Xiao, Yang Qingchuan*. Identification of alkali-responsive proteins from early seedling stage of two contrasting Medicago species by iTRAQ-based quantitative proteomic analysis[J]. Environmental and Experimental Botany, 2019, 157:26-34.

　　10. Long Ruicai, Li Mingna, Zhang Tiejun, Kang Junmei, Sun Yan, Cong Lili, Gao Yanli, Liu Fengqi, Yang Qingchuan*. Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress[J]. Frontiers in plant science, 2016, 7: 424.

　　11. Long Ruicai, Li Mingna, Li Xiao, Gao Yanli, Zhang Tiejun, Sun Yan, Kang Junmei, Wang Tenghua, Cong Lili, Yang Qingchuan*. A novel miRNA sponge form efficiently inhibits the activity of miR393 and enhances the salt tolerance and ABA insensitivity in Arabidopsis thaliana[J]. Plant molecular biology reporter, 2017, 35(4): 409-415.

　　12. Cong Lili, Sun Yan, Long Ruicai, Kang Junmei, Zhang Tiejun, Li Mingna, Wang Zhen, Yang Qingchuan*. Modulation of protein expression in alfalfa (Medicago sativa L.) root and leaf tissues by Fusarium proliferatum[J]. Journal of integrative agriculture, 2017, 16(11): 2558-2572.

　　13. Cong Lili, Sun Yan, Wang Zhen, Kang Junmei, Zhang Tiejun, Yang Qingchuan*. A rapid screening method for evaluating resistance of alfalfa (Medicago sativa L.) to Fusarium root rot[J]. Canadian journal of plant pathology, 2018, 40(1): 61-69.

　　14. Long Rruicai, Li Mingna, Kang Junmei, Zhang Tiejun, Sun Yan, Yang Qingchuan*. Small RNA deep sequencing identifies novel and salt‐stress‐regulated microRNAs from roots of Medicago sativa and Medicago truncatula[J]. Physiologia plantarum, 2015, 154(1): 13-27.

　　15. Long Ruicai, Zhang Fan, Li Zhenyi, Li Mingna, Cong Lili, Kang Junmei, Zhang Tiejun, Zhao Zhongxiang, Sun Yan, Yang Qingchuan*. Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula[J]. Journal of plant research, 2015, 128(4): 697-707.

　　16. Tong Zongyong, Quan Guoling, Wan Liqiang, He Feng*, Li Xianglin*. The effect of fertilizers on biomass and biodiversity on a semi-arid grassland of northern China. Sustainability, 2019, 11, 2854.

　　17. Li Zhenyi, Xu Hongyu, Li Yue, Wan Xiufu, Ma Zhao, Cao Jing, Li Zhensong, He Feng, Wang Yufei, Wan Liqiang, Tong Zongyong*, Li Xianglin*. Analysis of physiological and miRNA responses to Pi deficiency in alfalfa (Medicago sativa L.).Plant Molecular Biology. 2018, 96: 473-492

　　18. Feng He, Kun Wang, David B. Hannaway, Xianglin Li*, Effects of precipitation and clipping intensity on net primary productivity and composition of a Leymus chinensis temperate grassland steppe, Plos One, 2018, 43463:1-11.

　　19. He Feng, Tong Zongyong, Wang Lixue, Zheng Guiliang, Li Xianglin*, Effect of Fertilizer Additions on Plant Communities and Soil Properties in a Temperate Grassland Steppe, Polish Journal of Environmental Studies, 2018, 27(4): 1~8

　　20. Feng He, Kaiyun Xie, Xianglin Li*, Effect of Nitrogen Fertilizer and Seeding Rate on Yield of Alfalfa and Weeds, Polish Journal of Environmental Studies,2018, 27(2): 647-653

　　21. Li Yue, Wan Liqiang, Bi Shuyi, Wan Xiufu, Li Zhenyi, Cao Jing, Tong Zongyong, Xu Hongyu, He Feng, Li Xianglin*, Identification of Drought- Responsive MicroRNAs from Roots and Leaves of Alfalfa by High-Throughput Sequencing, Genes, 2017, 8,119; doi:10.3390/genes804011.

　　22. Liu Guixia, Wan Liqiang, He Feng, Tong Zongyong, Liu Zhongkuan, Li Xianglin*, Effects of litter, seed position, and water availability on establishment of seedlings for two semiarid grass species, Plant Ecology, 2016, 217(3): 277~287.

　　23. Gamal M.A.Fadul, Liqiang Wan, Feng He, Kaiyun Xie, Yan Qin, Xianglin Li*, Abscisic acid content in roots and root characteristics of alfalfa under deficit irrigation, African Journal of Agricultural Research, 2016, Vol.11(11),pp.935-940

　　24. Xie Kaiyun,Li Xianglin,He Feng, Zhang Yingjun, Wan Liqiang, David B Hannaway, Wang Dong, Qin Yan, Gamal M A Fadul, Effect of nitrogen fertilization on yield, N content, and nitrogen fixation of alfalfa and smooth bromegrass grown alone or in mixture in greenhouse pots, Journal of Integrative Agriculture, 2015, 14(9): 1864–1876

　　三、团队成员